Hi! I have problem with the pulldown assays. I've been trying to do Rho, Rac, cdc42 pull down assays but is not working. i see the GST band in the gel and few bands below that but nothing around 21KDa. Could you please help! Thanks Bee http://biowww.net/mynews/tree.php?group_name=bionet_molbio_proteins&begin=0