FLAG tagged Proteins/Xenopus

Ralf Rinke ralf.rinke at physiologie2.med.uni-erlangen.de
Mon Aug 2 06:53:03 EST 2004


Hi to all,

we are trying to detect FLAG - tagged (DYKDDDDK)proteins, which were
expressed in Xenopus oocytes by injecting them with cRNA for this proteins.
Detecting with SIGMA's Anti-Flag M2 Antibody, we receive a additional,
unspecific band at about 40 kDa in size.
Has anyone a hint, if this is a known problem in Xenopus?

Greetings
Ralf





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