Negative gel mobility shift

Saulius susirasom at takas.lt
Mon Aug 2 10:12:36 EST 2004


Hi all,

I was wondering if anyone has experience with NEGATIVE gel mobility shift?
Specifically, protein binds to RNA and instead of moving slower in native
gel runs significantly faster (!) than the free RNA probe. So far, Internet
search of similar stuff was unsuccessful. Any links to already known issues
or ideas how could it happen? Substrate is too short to have several
dramatically different conformations - 21nt. When replying, please CC to
mailbox. Many thanks

Saulius

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