correlation of IC50 to receptors/enzyme molecules

Louis Hom lhom at OCF.Berkeley.EDU
Fri Feb 6 08:33:31 EST 2004

In article <20040206003727.30725.qmail at>,
 <interpreneur_org at> wrote:
>In a binding or kinetic assay, how does the IC50 of a compound change
with respect to the number of receptors/enzyme molecules available? Is it
linear (e.g: 10 times more protein leads to a 10 fold increase in the
IC50)? Or not? And what is the mathematical equation explaining this?

	Does the affinity/dissociation constant of the receptor/enzyme 
change with the concentration of the receptor/enzyme?  That is, as you add 
more or less receptor/enzyme, does the binding energy between a receptor 
molecule and an inhibitor molecule change?
Lou Hom >K'93			     
lhom at 	    

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