Protein-Protein Interaction by Native Gel

[Philipp Pagel]

lcrowley at hsc.usf.edu wrote:
> I am currently working on a project showing the enhanced binding of
> two proteins via site directed mutagenesis and would like to visualize
> this by Native Gel.

My first question would be: Why did you choose to demonstrate the
interaction this way? Personally, I'd rather use co-IP, GST-pull-down or
something like this.

cu
	Philipp

-- 
Dr. Philipp Pagel                           Tel.  +49-89-3187-3675
Institute for Bioinformatics / MIPS         Fax.  +49-89-3187-3585
GSF - German National Research Center for Environment and Health
Email: p.pagel AT gsf . de
http://mips.gsf.de/~pagel