Protein-Protein Interaction by Native Gel

Louis Hom lhom at OCF.Berkeley.EDU
Wed Feb 11 11:48:57 EST 2004


I was also going to suggest gel filtration as an alternative, but then I 
realized that the problem you may be having is that the on-off rates may 
be causing your complex to get separated to bits in PAGE and probably 
would have the same problem in gel filtration.  How do you detect your 
proteins?  Are you just starting with two purified proteins and seeing if 
they bind to each other or is it a complex mixture like a cell lysate?
-- 
______________________________________________________________________________
Lou Hom >K'93			     
lhom at ocf.berkeley.edu		
http://www.ocf.berkeley.edu/~lhom/ 	    



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