Protein-Protein Interaction by Native Gel

P.C. PC at
Wed Feb 11 21:50:20 EST 2004

looks like a perfect project to try BiaCore ... (no aff., of course).

lcrowley at wrote:
> I am currently working on a project showing the enhanced binding of two proteins via site directed mutagenesis and would like to visualize this by Native Gel. I have made a poor attempt at this using a protocol from Sigma-Aldrich on a 12.5% pre-cast Bio-rad gel and my proteins completely separated on the gel. Are there any protocols out there that I could use that would help me with this, perhaps by lowering the ionic strength of the running and/or sample buffers.

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