expression periplasm

jocelyne matgelin jocelyne.mathelin at u-bourgogne.fr
Fri Feb 13 08:50:37 EST 2004


I try to espress a 18KD protein (from calycins family) in pET-22b
vector with BL21(DE3) strain, in order to obtain the protein of
interest in the periplasmic space (for disulfide bonds formation).
After several esperiments, I obtain my protein only in inclusion
bodies.
I tried different temperatures, with or without induction with IPTG at
different concentrations, short or long time culture.
What can I do now?
Thanks



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