Enzyme-friendly fixative

Louis Hom lhom at OCF.Berkeley.EDU
Sat Feb 14 20:11:25 EST 2004


In article <c0mdh5$18haje$3 at ID-140940.news.uni-berlin.de>,
Robert Goodman <robgood at bestweb.net> wrote:
>"Louis Hom" <lhom at OCF.Berkeley.EDU> wrote in message
>news:c0l6r1$1gtf$1 at agate.berkeley.edu...
>
>> Can anyone suggest a reagent for fixing cells that will preserve native
>> enzyme activity?
>> --
>Hard even to imagine such a thing!  However, perhaps one could preserve, not
>an entire cell nor even a section, but a sort of "ghost" of one, contents
>immobilized on a substrate like NC or in a gel.

I guess maybe I should have asked for something more like a tissue
fixative than cellular -- that is, I don't really care about subcellular
structures.  I guess maybe I should see how they fix specimens for b-gal
detection.  [oops, did I just ask a really dumb question?  oh well.]
-- 
______________________________________________________________________________
Lou Hom >K'93			     
lhom at ocf.berkeley.edu		
http://www.ocf.berkeley.edu/~lhom/ 	    



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