Alternatives to Laemli?

ahernan at cica.es ahernan at cica.es
Mon Feb 16 11:43:51 EST 2004


Hi all,

I am trying to run a protein-RNA complex on a SDS-PAGE gel but I was 
told that laemli's high pH can promote RNA hydrolysis. Any one knows 
of an alternative buffer system for protein SDS-PAGE at (near) 
neutral pH? I know Novex sells them as precast (and so do BioRad and 
Amersham...so it must not be under a patent) but buying such 
expensive stuff at this stage is out of the question.
Thanks a lot.
Cheers,
Agustin
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