Alternatives to Laemli

ahernan at cica.es ahernan at cica.es
Tue Feb 17 12:35:26 EST 2004


Thanks to all,

I think I'll try Mike Sullivan's suggestion but with a stacking on 
top (maybe 3% ).
As for the presence of SDS, the protein and the RNA are (hopefully!) 
crosslinked, so, if there are no problems with size, the complex 
should enter the gel and stay put.
Anyone heard about a Tris-Acetate system, anyway?
Cheers

Agustin
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