protocol for methionine incorporation

viegas at viegas at
Sun Jan 11 10:16:36 EST 2004

I wonder if someone has a protocol for [35S]methionine incorporation to be used 
in Hela cells. In particular, I am interested in the amount of uCi to use per 
ml. and per number of cells. Secondly, I´d like to now if the cells have to be 
in confluence for this essay or not. Thank you very much in advance,
                                        Marcelo Viegas


More information about the Proteins mailing list