what class compound it can be?!

wujien wujien at imcb.a-star.edu.sg
Thu Jun 24 04:34:56 EST 2004


Hi everyone:

I'm isolating a signal molecule from a fungus' culture. The MW of the
molecule should be no more than 1000 Da. The molecule is water soluble
and can not be extracted by organic solvent. It also can not binding
with ion-exchanged resins (except strong acid ones; however, it is
sensitive to strong base). I have to use sephadex G10 and G15.
Unfortunate, it is seemed that the molecule binding with the sephadex
(activity lost >90%). Who can give me some advices to resolve this
problem.

Sincerely
Wu Jien



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