white out

袖岡 msd at ior.twmu.ac.jp
Tue Jun 29 04:14:44 EST 2004


Hello.


 My problem is the 32kDa protein is supposed to be there,

and other experiments, RT-PCR, IHC, and DNA ladder assay

suggest the exhistance of the protein, but the objective band

in western blot , though it is supposed to be black on X-ray

film,  is a white band just like a ghost in the night. Is there anyone

who has the right method to avoid this failure?  I used  goat IgG

for first antibody, and second antibody was Rabbit IgG anti

goat IgG.    Please Help me!


                                   

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