white out

[Dr. Hiranya S. Roychowdhury]

Not clear!

Quoting "$BB52,(J" <msd at ior.twmu.ac.jp>:

> Hello.
> 
> 
>  My problem is the 32kDa protein is supposed to be there,
> 
> and other experiments, RT-PCR, IHC, and DNA ladder assay
> 
> suggest the exhistance of the protein, but the objective band
> 
> in western blot , though it is supposed to be black on X-ray
> 
> film,  is a white band just like a ghost in the night. Is there anyone
> 
> who has the right method to avoid this failure?  I used  goat IgG
> 
> for first antibody, and second antibody was Rabbit IgG anti
> 
> goat IgG.    Please Help me!
> 
> 
>                                    
> 
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> 


-- 
Hiranya S. Roychowdhury, Ph.D.
Coll. Asst. Professor,
Molecular Biology,
Dept. of Chemistry & Biochemistry
Rm# 336, Chemistry Bldg.; MSC 3MLS
New Mexico State University
Las Cruces, NM 88003
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