Protein purification problem

Sulakshana Mukherjee mukherji at tifr.res.in
Fri Mar 12 23:02:24 EST 2004


Hello!
Use Mass Spectroscopy to determine your protein's Mol. Wgt. This can
also give the purity of your sample. You can as well use capillary
electrophoresis for the purpose.

with best regards,
Sulakshana

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Sulakshana Mukherjee
Research scholar
Molecular Biophysics Group
Tata Institute of Fundamental Research
Homi Bhabha Road
Mumbai-400005
India
Phone: +91(22)22804545 ex-2271
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On Sat, 12 Mar 2004 gilbertjack at hotmail.com wrote:

>
> I am currently working on the most difficult project I have ever seen and have finally been forced to take the plunge and ask for outside assistance.
>
> This protein is a novel type from bacteria. Size exclusion chromatography suggests it to be 35-45kDa. It does not stain by coomassie, silver stain or any traditional techniques on SDS-PAGE. However, it is stained by Stains-All, but this shows it to be stuck at the stacking/resolving gel interface.
>
> We have tested for amino-sugars and found none.
>
> Does anyone have any idea why a protein would not travel through a polyacrylamide gel at all?
>
> If anyone has noticed anything like this before I would love to speak with you in more detail.
>
> Yours sincerely,
>
> Jack Gilbert
>
> http://biowww.net/mynews/tree.php?group_name=bionet_molbio_proteins&begin=0
>
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