how to concentrate protein in SDS sample buffer
dreamwestus at yahoo.com
Mon Mar 15 10:53:28 EST 2004
I am trying to do a co-IP experiment on a HA tagged protein, and I eluted
the protein with SDS buffer. However, the protein level is very low that
after I ran the eluate on a gel followed by silver staining, I couldn't see
any band. So now I am thinking combining all the eluate from multiple
experiments. Is there a way that I can concentrate all these proteins?
Will TCA precipitation work in the presence of SDS? Or is there any kind of
concentrator that I can use? Thank you very much!
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