How to extract protein from the SDS gel

P.C. PC at nospam.edu
Sat Mar 27 15:22:41 EST 2004


what is downstream - mass spectrometry or something else? If it is MS, 
then I would avoid using SDS in extraction - there are couple of methods 
papers describing isopropanol/acetonitrile/water solutions for that.
I can look up more specific details, if you need.

Peter

qianxf at bu.edu wrote:
> Hello, 
> 
>     Is there somebody know how I can extract the protein from the SDS gel to the solution without digesting it?
> 
>    Thanks a lot.
> 
> http://biowww.net/mynews/tree.php?group_name=bionet_molbio_proteins&begin=0




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