How to extract protein from the SDS gel

Dr Engelbert Buxbaum engelbert_buxbaum at hotmail.com
Sun Mar 28 02:39:40 EST 2004


qianxf at bu.edu wrote:

>     Is there somebody know how I can extract the protein from the SDS
> gel to the solution without digesting it?


Electroelution. Check the manufacturer of your electrophoresis equipment
for matching electroelution tubes. 

The gel is Coomassie-stained, the band of interest cut out and finely
chopped. The bits are held by a nylon net inside the tubes, the bottom
is closed of with dialysis membrane. The apparatus is filled with
transfer buffer (either Towbin or Dunn). Both proteins and coomassie
collect in a small volume of liquid on top of the membrane.



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