How to extract protein from the SDS gel
Dr Engelbert Buxbaum
engelbert_buxbaum at hotmail.com
Sun Mar 28 02:39:40 EST 2004
qianxf at bu.edu wrote:
> Is there somebody know how I can extract the protein from the SDS
> gel to the solution without digesting it?
Electroelution. Check the manufacturer of your electrophoresis equipment
for matching electroelution tubes.
The gel is Coomassie-stained, the band of interest cut out and finely
chopped. The bits are held by a nylon net inside the tubes, the bottom
is closed of with dialysis membrane. The apparatus is filled with
transfer buffer (either Towbin or Dunn). Both proteins and coomassie
collect in a small volume of liquid on top of the membrane.
More information about the Proteins