GST expression problem

Huey-Jen Fang hueyjen at gmail.com
Thu Sep 16 22:55:06 EST 2004


I used two types of GST expression vectors(pET42b and pGEX) to express
my protein and I had cloned my protein gene in pET42b successfully.
However, I found that this construction can't express my protein after
IPTG induction and then I tried to express the pure pET42b vector to
find out the problem. Unfortunately, I did not observe any GST protein
expression after induction in BL21, BL21-DE3 and BL21-DE3-pLYS
strains.If any one had meet the same problem likes my case, please
give me some suggestions to improve my experiment.

Thank You 

Huey-Jen Fang



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