homemade prestained proteins...

peter willingmann bt8a002 at uni-hamburg.de
Mon Sep 20 07:57:54 EST 2004


Hi Netters,
i would like to make homemade prestained protreins for SDS-PAGE. I have 
found a method wich used Remazol Dye and 50 mM Tris/038M Glycine 
described from T.S. Piallay.. In my hand its not working properly
e.g. the stain is not intense or the labelling efficency of the dye to 
the proteins is not effective... I was using BSA, Ovalbumin, Lysozyme etc..
Do anybody have a better method or could give me a tip...!!??
Thanks in advance
peter



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