homemade prestained proteins...
bt8a002 at uni-hamburg.de
Mon Sep 20 07:57:54 EST 2004
i would like to make homemade prestained protreins for SDS-PAGE. I have
found a method wich used Remazol Dye and 50 mM Tris/038M Glycine
described from T.S. Piallay.. In my hand its not working properly
e.g. the stain is not intense or the labelling efficency of the dye to
the proteins is not effective... I was using BSA, Ovalbumin, Lysozyme etc..
Do anybody have a better method or could give me a tip...!!??
Thanks in advance
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