[Protein-analysis] Re: protein purification for immunization?
C.Fritsch at DKFZ-Heidelberg.de
Fri Aug 26 02:26:32 EST 2005
"Sonja Kowalczuk" <sweepthedoghouse at hotmail.com> schrieb im Newsbeitrag
news:mailman.412.1124907571.29381.proteins at net.bio.net...
>I have created GST-fusion proteins and purified them on glutathione
>sepharose 4B columns, so the protein was eluted with 10mM glutathione in
>50mM Tris-HCl pH7.5.
> I want to use the fusion proteins to immunize some rabbits.
> Do I need to get rid of the Tris to have the protein in water only (and if
> so how do I do this)?
> Thank you
I would suggest dialyisis against PBS.
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