[Protein-analysis] Re: how to make protein unfold curve

subberry zhangpy at gmail.com
Mon Aug 29 08:35:51 EST 2005


 ^
 |          oxoxoxox
 |        o x
 |     o  x
 |    o  x
 |   o  x
 | xox x
 +------------------------>
          c(GdnHCl)/M

In this diagram, "x" and "o"representing denature and renature process
respectively, is it right?For "x" we can do it by dissolve the nature
protein in the buffers with varying GdnHCl concentrations, and for "o"
we can  denature the protein in high GdnHCl ,and Then dilute it into
the buffers with varying
GdnHCl concentrations, So my question is how long can we measure after
dissovling or dilute? is it we should wait the refolding (unfolding)
reach equilibium?



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