[Protein-analysis] Re: trypsin digestion

[Kyle Legate]

nithya wrote:
> I have done a trypsin digestion of a coloured protein.........
> my protocol is 1mg/ml trypsin in 0.1% acetic acid.......
> protein soln 40 ug/ml in 100 mM AmBic buffer ph:8.5
> overnight incubation
> prot: trypsin ratio :  100:1/ 50:1
> 
> my boss says that i need to c a reduction in color of the prot if
> digestion has occurred.....
> 
> is ther any other way to confirm that digestion has occurred
> completely?
> 
> I would welcome any suggestions on the protocol as well
> 
> have to do MS of the digested peptides
> 
> help me
> 
> thanks
> 
> Nithya
> 
Run an SDS-PAGE gel--does your full-length protein disappear? You'll 
have to run a gel before MS anyway.