[Protein-analysis] Re: Enzyme immobilization.

Krishnan Chittur kchittur at athena.eb.uah.edu
Mon Jul 25 10:36:01 EST 2005


Chris B. <chris.b.nospam at nospam.interia.pl> wrote:
> "Dr Engelbert Buxbaum" wrote:
>> Unfortunately, pre-activated gels (either CNBr or other chemistries) are
> not cheap.
> 
> I know about CNBr pre-activated gels and their prices, that's why I was
> searching for something cheaper.
> 
> Chitosan + glutaraldehyde worked fine for me.
 
To ask a related question ... other than the CNBr activation method,
what are some alternatives?  (that is, if I want to prepare 
a DEAE-sepharose using DEAE and sepaharose - what are some methods
that will work??) (will not glutaraldehyde mess with protein structure
if you are using it to immobilize it onto a matrix like chitosan?)

 


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