[Protein-analysis] Re: Enzyme immobilization.
Dr Engelbert Buxbaum
engelbert_buxbaum at hotmail.com
Wed Jul 27 01:20:03 EST 2005
Krishnan Chittur wrote:
> (will not glutaraldehyde mess with protein structure
> if you are using it to immobilize it onto a matrix like chitosan?)
Of course it will, by making cross-links both between protein and matrix
and (depending on method) between proteins. That is the purpose of the
method. The other question is to what extend this will affect the
properties of the protein (enzyme activity, ligand binding).
Unfortunately, this can be answered only empirically.
More information about the Proteins