[Protein-analysis] Re: Enzyme immobilization.

Louis Hom lhom at OCF.Berkeley.EDU
Wed Jul 27 09:36:10 EST 2005


One alternative that has not been mentioned (but that I don't have any 
personal experience with yet) is to use carbonyl diimidazole to activate 
-OH groups on your resin, for reaction with primary amines in your 
protein.  I believe the CDI activation has to be done in the absence of 
water, but that the immobilization occurs under typical amine-related 
conditions (e.g., pH 8 using a non-nucleophilic buffer).

There may also be some carboxylate-based cation exchange resins out there 
(e.g., sephadex CM) that you could activate with NHS.
-- 
______________________________________________________________________________
Lou Hom >K'93			     
lhom at ocf.berkeley.edu		
http://www.ocf.berkeley.edu/~lhom/ 	    


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