[Protein-analysis] Re: separate proteins of 66K and 70K

[Dr Engelbert Buxbaum]

rocky wrote:

> Hi All
> 
> For western blot, is it possible to separate proteins of size 66kDa and
> 70kDa using gradient gel? What percentage of gel should be used?

I have done something like that once with Hsc70 on a 5-10% gradient gel.
You may also whish to check for the use of Na-tetradecylsulphate as
detergent, which allowed the separation of Na/K-ATPase isoforms that
could not be separated in the presence of SDS.