[Protein-analysis] Re: heterooligomer or homooligomer

SL user at eamples.net
Thu Nov 17 17:59:02 EST 2005


I mean to know this before I am starting to purify this complex.

thanks.

ls

SL wrote:
> Hi,
> 
>  From gel filtration + western, my protein looks much bigger (~ 200 kD) 
> than its monomer size (~60 kD). I am just wondering if there is a method 
> I can detect if it's a protein complex (with other proteins), or just a 
> oligomer of itself.
> 
> Any suggestions are welcome.
> 
> Thanks.
> 
> LS


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