[Protein-analysis] Re: heterooligomer or homooligomer

Dr Engelbert Buxbaum engelbert_buxbaum at hotmail.com
Sat Nov 26 03:03:10 EST 2005

botulina at poczta.onet.pl wrote:

> "SDS under non-denaturing conditions" the most beautiful oxymorone I've
> ever heard.

Not really. If the sample is gently heated in the presence of SDS, but
absence of sulphhydryls, some (but not all) protein secondary and
tertiary structure as well as protein-proteins interactions are
maintained. Of course, even milder  methods are also available, DISK-
and native-blue electrophoresis are but two. Indeed, it is possible to
do a non-denaturing electrophoresis in the first and a denaturing in the
second dimension. This is a very useful method to study protein-protein

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