[Protein-analysis] Gly4-Ser linker proteolytic degradation

patrick.stancombe at hpa.org.uk patrick.stancombe at hpa.org.uk
Thu Sep 15 07:50:28 EST 2005


E.coli expression of a two domain protein is undergoing specific
proteolytic cleavage at the (Gly4-Ser)x4 linker site between them.
Tried using protease inhibitor cocktails without success. Is this a
common problem? Does anyone have suggestions to what is the cause and
what can be tried to prevent it?
Thanks



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