[Protein-analysis] Re: affinity purification of polyclonal antisera

StewJW stewjw at gmail.com
Wed Aug 9 08:12:09 EST 2006


What kit are you refering to?

If you need to bind your protein to a solid matix for affinity
purification the easiest method is to use CNBr activated sepharose.

http://www4.amershambiosciences.com/aptrix/upp01077.nsf/Content/Products?OpenDocument&parentid=5073&moduleid=11872&zone=Labsep

The Amersham fast flow is better but the 4B version maybe cheaper and
adequate for small scale.



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