[Protein-analysis] Re: Protein Solubility Problem

[J Rice]

Dr Engelbert Buxbaum wrote:

> I am not sure why you included the Tris in the first place, imidazole is
> a perfectly good buffer.

It wasn't completely by choice -- all the buffers for the Novagen
His-bind kit are Tris based.  It would not be a problem to make them
without Tris, but obviously I was not anticipating this problem with
the dialysis.