[Protein-analysis] Ammonium bicarbonate in IE chromatography

pawel.stocki at ncl.ac.uk pawel.stocki at ncl.ac.uk
Thu Aug 31 05:26:49 EST 2006


Hi,
Does anyone have some experience in using ammonium bicarbonate buffer
for gradient elution from Q-Sepharose or other IE? I would like to use
it instead of NaCl gradient. The sample will be subjected for further
MS analysis that is why I would like to use volatile salt. I'll
appreciate any comments. 

Thanks,
Pawel



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