[Protein-analysis] Re: regarding immunoprecipitation

[Dr Engelbert Buxbaum]

Ramachandran Balasubramanian wrote:

> hello everyone,
> 
> I have been working on immunoprecipitation ie pull-down of my protein
> using a monoclonal antibody 
> 
> Can anyone suggest a good procedure for pull-down assays. or is my protein
> interaction has some problem.

Calbiochem sells Pansorbin, killed Staph. aureus cells expressing
protein A on their surface. They also make a nice booklet on its use
with lots of recipes. Handy even if you prefere protein A agarose over
Pansorbin.