[Protein-analysis] protease

Abla Sipanis via proteins%40net.bio.net (by ablasipanis At yahoo.co.uk)
Mon Dec 11 10:20:49 EST 2006


Hi all,

I'm looking for a protease that has a very specific cleavage site, and 
quite long so that it wouldn't cut at all in the whole Arabidopsis 
proteome. Do you think that this does exist ?
Amersham for example claims that the recognition site for Prescission is 
LeuGluValLeuPheGln/GlyPro. Does it mean that this is really the only 
sequence that will be cut ? And by the way, I would like to express this 
protease in vivo, so that I would need a cDNA. Perhaps difficult for a 
patented enzyme ?

Any suggestion would be ighly appreciated.
Thanks for you help !

Abla


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