[Protein-analysis] Basic question about PMSF

Breslauer via proteins%40net.bio.net (by breslauer1981 At gmail.com)
Wed Dec 13 12:05:59 EST 2006


Listen,
is it normal that when adding PMSF (250mM solution in MeOH) to cell
free extract from E.coli (broked by sonication and spinned 12000g for
30min) to the final concentration of 0,5mM buffer becomes cloudly and
st become to precipitate (probably protein?).

Thans for any clue

Julio



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