[Protein-analysis] AW: Proteins Digest, Vol 19, Issue 6

nschmitz At freesurf.ch via proteins%40net.bio.net (by nschmitz At freesurf.ch)
Thu Dec 14 08:18:28 EST 2006


Ad Polytron homogeniser Kinematica

I used this apparatous already, but never disassembled it. I only released
the generator by pushing a buttom and turning the generator. Before removing
it, we used to clean the generator by running the homogenizer in a 10 % SDS
solution, followed by running it in a 70% ethanol solution. Because of aerosol,
I placed the homogeniser into a fume hood to run it. The homogeniser can
be autoclaved as described on the company?s homepage under FAQ: http://www.brinkmann.com/faqs/Homogenizers.asp

Sincerely

Dr Nicole MR Schmitz

>-- Originalnachricht --
>Date: Tue, 12 Dec 2006 12:01:16 -0500 (EST)
>From: proteins-request At oat.bio.indiana.edu
>Subject: Proteins Digest, Vol 19, Issue 6
>To: proteins At magpie.bio.indiana.edu
>Reply-To: proteins At oat.bio.indiana.edu
>
>
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>Today's Topics:
>
>   1. Dismantling Polytron EasyCare Homogeniser (IanMc)
>   2. Re: protease (ChenHA)
>
>
>----------------------------------------------------------------------
>
>Message: 1
>Date: Mon, 11 Dec 2006 13:10:08 -0000
>From: "IanMc" <zebedeeboy At hotmail.com>
>Subject: [Protein-analysis] Dismantling Polytron EasyCare Homogeniser
>To: proteins At net.bio.net
>Message-ID: <mailman.390.1165873983.19683.arrays At net.bio.net>
>
>Hi,
>
>I am getting nowhere fast with the companies who sold me our Polytron 
>homogeniser. Does anyone know how to take apart a Kinematica/Brinkman 
>Polytron homegeniser generator for cleaning? We are using it on gut samples
>
>and sheets of connective tissue are getting trapped between the rotor and
>
>stator. They ship a 'tool' with the generator but without any instructions
>
>on how to use it.
>
>Anybody out there who has overcome this particular problem?
>
>TIA,
>
>Ian Mc 
>
>
>
>
>------------------------------
>
>Message: 2
>Date: Mon, 11 Dec 2006 22:26:06 +0000
>From: ChenHA <hzhen At freeuk.com>
>Subject: [Protein-analysis] Re: protease
>To: proteins At net.bio.net
>Message-ID: <qglrn2h3vg0jnpquthmuhm952tdnnn89ig At 4ax.com>
>Content-Type: text/plain; charset=us-ascii
>
>On Mon, 11 Dec 2006 16:20:49 +0100, Abla Sipanis
><ablasipanis At yahoo.co.uk> wrote:
>
>>Hi all,
>>
>>I'm looking for a protease that has a very specific cleavage site, and

>>quite long so that it wouldn't cut at all in the whole Arabidopsis 
>>proteome. Do you think that this does exist ?
>>Amersham for example claims that the recognition site for Prescission is
>
>>LeuGluValLeuPheGln/GlyPro. Does it mean that this is really the only 
>>sequence that will be cut ? And by the way, I would like to express this
>
>>protease in vivo, so that I would need a cDNA. Perhaps difficult for a

>>patented enzyme ?
>
>I very much doubt that it would cut only at that specific sequence.
>You can check the literature.  Prescission is actually 3C protease
>from human rhinovirus, so have a look.  If I remember correctly, it
>can cleave a number of different sequences.
>
>We make our own 3C protease from HRV 14 although I'm not sure if it is
>the exact same one as Prescission (not really sure if there are
>different kinds of 3C proteases from HRV, have a look in the
>literature).  The one we use belongs to another lab, so can't offer it
>to you myself, but it's something you can clone it yourself.   It  is
>fused to GST in pGEX and needs to be expressed at low temperature (18
>to 20 deg C) or else it would form inclusion body.
>
>
>
>
>>
>>Any suggestion would be ighly appreciated.
>>Thanks for you help !
>>
>>Abla
>
>
>
>------------------------------
>
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>End of Proteins Digest, Vol 19, Issue 6
>***************************************


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