[Protein-analysis] protein estimation.

sarita malik sarita2509 at rediffmail.com
Fri Feb 24 06:31:40 EST 2006


  
  i have a very simple Query
how do u estimate protein concentration of a whole cell protein sample denatured in lammeli buffer for performing PAGE. i tried Lowrys method and Brad ford but both methods gave inconsistent results. when compared compared with electrophoresis of a known protein sample.

thanx

Sarita  


Sarita Mallik
Ph.D Scholar
C/o Dr. J.S. Virdi
Microbial Pathogenicity Laboratory
Dept. of Microbiology
Universitty of Delhi South Campus
Benito Juarez Road
New Delhi - 110 021
India


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