[Protein-analysis] Re: protein estimation.

Nick Theodorakis nick_theodorakis at hotmail.com
Fri Feb 24 20:29:12 EST 2006


sarita malik wrote:
>   
>   i have a very simple Query
> how do u estimate protein concentration of a whole cell protein sample denatured in lammeli buffer for performing PAGE. i tried Lowrys method and Brad ford but both methods gave inconsistent results. when compared compared with electrophoresis of a known protein sample.
> 


SDS interferes with the Bradford assay. It's been years since I've done 
a Lowry, so I can't remember if it can tolerate it.

However, you can use the Pierce BCA assay, but not if you have reducing 
agents in your sample.

Nick

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