[Protein-analysis] Re: protein estimation.

Allison allison at nospam.com
Sat Feb 25 09:38:23 EST 2006


Nick Theodorakis wrote:
> sarita malik wrote:
> 
>>     i have a very simple Query
>> how do u estimate protein concentration of a whole cell protein sample 
>> denatured in lammeli buffer for performing PAGE. i tried Lowrys method 
>> and Brad ford but both methods gave inconsistent results. when 
>> compared compared with electrophoresis of a known protein sample.
>>
> 
> 
> SDS interferes with the Bradford assay. It's been years since I've done 
> a Lowry, so I can't remember if it can tolerate it.
> 
> However, you can use the Pierce BCA assay, but not if you have reducing 
> agents in your sample.
> 
> Nick
> 
there is a protein assay reagent from Biorad that is compatible with 
Laemmli buffer - "RC DC Protein Assay"

Allison


More information about the Proteins mailing list