[Protein-analysis] Re: protein estimation.
allison at nospam.com
Sat Feb 25 09:38:23 EST 2006
Nick Theodorakis wrote:
> sarita malik wrote:
>> i have a very simple Query
>> how do u estimate protein concentration of a whole cell protein sample
>> denatured in lammeli buffer for performing PAGE. i tried Lowrys method
>> and Brad ford but both methods gave inconsistent results. when
>> compared compared with electrophoresis of a known protein sample.
> SDS interferes with the Bradford assay. It's been years since I've done
> a Lowry, so I can't remember if it can tolerate it.
> However, you can use the Pierce BCA assay, but not if you have reducing
> agents in your sample.
there is a protein assay reagent from Biorad that is compatible with
Laemmli buffer - "RC DC Protein Assay"
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