[Protein-analysis] Re: Protease essay

Dr Engelbert Buxbaum engelbert_buxbaum at hotmail.com
Tue Jul 18 02:18:55 EST 2006


daniella schatz wrote:

> Hello all,
> When running an "In gel protease essay", one can see clear bands where the
> protease has been active. This is due to the protease degrading the
> substrate that is in the gel. How is it that the stain (usually Coomassie)
> does not stain the actual protease? Also, can the protease be excised and
> extracted from the gel after staining?

because an enzyme (protease) is present at much lower concentration than
the substrate (gelatin or whatever).

And yes, in principle at least it is possible to elute proteins from
stained gels, usually by electroelution. 


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