[Protein-analysis] PEGylated protein on SDS-PAGE

Muhammad Farooq Sabar farooqsabar at hotmail.com
Wed Jul 26 00:30:10 EST 2006


Hi every one!
My recombinant protein is of size 19KDa. After PEGylatiing it with 5KDa PEG 
(Polyethylene glycol) molecule what could be the position of PEGylated 
protein on 8% SDS-PAGE? Notice that PEGylated proteins have different size 
on SDS-PAGE from the sum sizes of PEG and Protein. Your response will be 
highly appreciated. Thank you.

Muhammad Farooq Sabar
Lahore-Pakistan

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