[Protein-analysis] 7kDa protein electrophoresis
dskerr at gmail.com
Thu Mar 9 13:05:21 EST 2006
I'm trying to detect a 7kDa protein by Western Blot.
I've ran the cell lysate in a 15% acrylamide gel and the problem is,
proteins bellow 14kDa just vanish.
The Molecular standard is broad range (6-150kDa) and even the weight
doesn't show in coomassie or in the ponceau (I've stained half the gel
and transfered the other half).
Does anyone has a tip or similar problem?
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