[Protein-analysis] 7kDa protein electrophoresis

Daniel Kerr dskerr at gmail.com
Thu Mar 9 13:05:21 EST 2006


I'm trying to detect a 7kDa protein by Western Blot.

I've ran the cell lysate in a 15% acrylamide gel and the problem is,
proteins bellow 14kDa just vanish.

The Molecular standard is broad range (6-150kDa) and even the weight
doesn't show in coomassie or in the ponceau (I've stained half the gel
and transfered the other half).

Does anyone has a tip or similar problem?

Thanks



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