[Protein-analysis] Re: DLS at high protein concentration

QCZ heat-capacity at indy.rr.com
Wed Mar 15 07:52:46 EST 2006


Zhao,
Thanks for your input. Sorry I did not indicate that I was using a
batch-mode ALV/CGS-3 machine and it has an attenuator.  So the flow rate and
signal saturation are not problems for me in this case. I believe that what
is measured is D(app). D(app)=D(0)*(1+k*c).  So extrapolation to c=0 would
give us the intrinsic diffusion coefficient that can be used to calculate
the intrinsic Rh. 
 
Regards,
 
Chin


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