[Protein-analysis] Re: 7kDa protein electrophoresis

Dr Engelbert Buxbaum engelbert_buxbaum at hotmail.com
Thu Mar 16 10:49:41 EST 2006


DK wrote:


> membranes combined with the removal of SDS from gel
> (15-30  min soak in transfer buffer before assembling 
> the sandwich) should help. May also try increasing MeOH
> in transfer buffer to 30%. 

And of course: leave out the SDS from the transfer buffer. SDS increases
mobilisation of large proteins, but reduces binding to the membrane.
MeOH increases binding of small proteins to the membrane, but reduces
mobility. Thus only one or the other are to be used, not both at the
same time, as commonly seen.


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