[Protein-analysis] Prestained SDS-PAGE markers WITHOUT reducing agent

DA da8688 at yahoo.com
Fri Mar 17 11:21:50 EST 2006


Dear all,
I am trying to find a company that sells prestained
SDS-PAGE markers without added reducing agent.  All of
the vendors I have found included reducing agents in
their products.  
Can someone point me in the right direction?
Thank you very much.
D. Andrews
University of Miami

--- Dr Engelbert Buxbaum
<engelbert_buxbaum at hotmail.com> wrote:

> DK wrote:
> 
> 
> > membranes combined with the removal of SDS from
> gel
> > (15-30  min soak in transfer buffer before
> assembling 
> > the sandwich) should help. May also try increasing
> MeOH
> > in transfer buffer to 30%. 
> 
> And of course: leave out the SDS from the transfer
> buffer. SDS increases
> mobilisation of large proteins, but reduces binding
> to the membrane.
> MeOH increases binding of small proteins to the
> membrane, but reduces
> mobility. Thus only one or the other are to be used,
> not both at the
> same time, as commonly seen.
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> Proteins at net.bio.net
> http://www.bio.net/biomail/listinfo/proteins
> 


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