[Protein-analysis] protein contamination with DNA???

nithya blueylife at gmail.com
Tue Mar 21 09:01:37 EST 2006


I am basically a chemist and do not know much about protein
purification. I am facing problems to purify a 15 KD metalloprotein
containing cobalt. even after passing through DEAE sepharose and
SEPHADEX-G 25, there is a broad band between 220-300 nm. SDS-PAGE gives
a single band. what could be the possible contaminant? since it is a
thermostable protein i have heated upto 75 deg to remove other
proteins. could you please help me out.



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