[Protein-analysis] Re: adding nucleases to SDS PAGE samples

Nick Theodorakis nick_theodorakis at hotmail.com
Sat Mar 25 21:20:44 EST 2006


DA wrote:
> What are the general opinions of the group regarding
> digesting tissue culture cell preparations with
> nucleases during sample prep for SDS-PAGE. If one
> doesn't add nucleases, are there physical or chemical
> strategies to make samples less viscous?
> Thanks for any thoughts.
> David

You could sonicate, or run the lysate through a small bore needle (e.g., 
25g -ish) and syringe a few times.

Nick


-- 
Nick Theodorakis
nick_theodorakis at hotmail.com
contact form:
http://theodorakis.net/contact.html


More information about the Proteins mailing list