[Protein-analysis] Batch vs column chromatography

Julio juliocortazar at o2.pl
Wed Mar 29 04:13:27 EST 2006


I'm puryfing a protein using Ni-NTA (Qiagen) technology. Recently I
load the crude extract from my protein onto the column, but I'm
considerating using of batch method. Tell me please if it is reasonable
to add about 1mL of resign to the 150mL of crude extract and leave it
vigorously shaking overnight? Are these proportions of volumes and
times are reasonable?


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